Background: elevated B-hCG level in cervicovaginal secretion may be considered as a marker to predict preterm labor. Aim: Aim of this prospective study was to assess the correlation between cervicovaginal levels of B-hCG and preterm labor. Methods: 120 pregnant women at gestational age 28-36 weeks, including 40 as control (group II) having no preterm labour pain and 80 in the study group having preterm labour pain of which 40 had preterm delivery (group Ia) and 40 had term delivery (group Ib). Results: The median of B-hCG level (mIu/ml) in group IA was 24.5(14-44.5), in Ib was 7.6(6-24.5), in II was 3.6(2.35-4.5), (p<0.001). The cut off value of β-hCG >5.8 mIU/ml in cervicovaginal secretion can be used to predict preterm delivery in women with preterm labor pain. Conclusions: β-hCG level in cervico-vaginal secretion can be used as a predictor of preterm labor in women presenting with preterm labor pain, with cut off value > 5.8 mIu/ml.