This article aims at studying, in more details, the possible causes and interpretations behind the development of antisperm antibodies in virgins. This descriptive case study included 35-years old single women with positive ASA. The mean serum antisperm antibody concentrations is (62.1 IU/ml), it is considered as positive titer. Complete blood count is normal, except for slight increase in WBC count and percentage of basophils, monocytes and lymphocytes. This study recorded high concentrations of serum total IgG and IgM levels (1870 IU/ml and 255 IU/ml respectively). The UTI was confirmed by counting total bacterial concentration (167220 CFU/ml) in the urine. There is two suggested mechanisms to explain ASA in virgins: (1) antigen cross-reactivity between sperm and bacterial antigens to which antibodies can react; () 2induction of the immune system by antigens of sperm ingested into the gastrointestinal tract with contaminated food and drink.